How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How to size battery banks?

I am designing a small grid connected solar PV power-plant. As the battery's capacity is specified in Ah and demand in kWh. What is the best way to size the battery bank? I have battery price in...

28 February 2021 1,638 3 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

What is the minimum number of studies in each category required for a multiple subgroup analysis to be viable in a meta-analysis?

Cochrane Handbook mentions subgroup analyses are only warranted if there are more than 10 studies (I have 28), and if they are distributed evenly among categories of subgroup. I am wondering if...

17 February 2021 3,467 4 View

How do you interpret multiple meta-regression in STATA?

Please could someone recommend a resource that explains performing meta-regression with multiple moderators. I have read the STATA META ANALYSIS REFERENCE MANUAL, but this only gives examples of...

17 February 2021 7,376 1 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

How do you incorporate a study that used non-parametric analysis into a meta-analysis where majority of studies used parametric?

I am calculating Hedge's g for effect sizes, so I am pulling means, SDs, and Ns, from each study. However, one study did a Mann-Whitney U test, and so reports Mean Rank, Sum of Ranks, U, Z, and r...

31 January 2021 1,182 5 View

What is better to buy a real-time pcr machine or an endpoint?

Hello everyone, we are acquiring a new molecular biology laboratory with the fundamental objective of identifying species of microalgae and cyanobacteria. Perhaps you could help us decide if it is...

24 January 2021 8,320 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Methodology for bird counts (census) in airport?

Hello. I am looking for a description of a methodology for bird counts (census), which is applicable to airport area and will allow me to collect the data about species diversity and abundance at...

11 January 2021 3,213 3 View

If anyone can suggest or review manuscript having keywords Riparian zone, River, Species richness, Species evenness, Ornithofauna?

Greetings I am Sachin Kumar, pursuing my Ph.D. in Ornithology, from PAU, Ludhiana. I am working on avian diversity along the River Beas. I had submitted a manuscript to a journal having keywords...

10 November 2020 7,194 6 View

Do you have an endnote style for Marine Ornithology?

Any seabird folks out there have an endnote style file for Marine Ornithology? Nothing available online, trying to avoid creating one from scratch - thanks! Perhaps we can make the file publicly...

01 November 2020 9,642 7 View

Methods for bird trend analysis?

What would be the best method for analysing trends in species abundance in a single area in Latin America over a 15 year period? Could TRIM be used for this purpose? I don't have data about...

27 October 2020 7,532 5 View

Does anyone have experience with movement-based kernel density estimation (MKDE)?

Hello, I have mapped the locations of several individuals of a certain species of bird. I have the GPS coordinates, the date and the time data every half hour for several consecutive days and I am...

26 September 2020 3,712 2 View

Are half duplex or full duplex PIT tags the standard in ornithological studies using RFID technology to track avian movements?

I am looking for the correct selection of tags to use to monitor tree swallows moving in and out of nest boxes. My impression is that full duplex tags would be more appropriate but many studies...

26 May 2020 4,647 4 View

What are the statistical methodologies currently available for combining mist-net and audio-visual surveying methods for birds?

While there are a number of studies that employ both constant effort mist-netting and audio-visual survey methods, there are very few that combine the numbers from those surveys into a single...

10 May 2020 8,432 1 View

Monitoring breeder/floater ratio in bird populations in relation to nest-site availability?

Hi all, I was wondering if you know of any case in which breeder/floater ratio in birds is monitored over the years while observing changes in nest-site availability. Floaters in one of my focus...

29 March 2020 8,213 28 View

Can we use Ebird Personal Checklists as standard Journal References?

05 March 2020 2,002 7 View