Dear Ahmed,
First, you have to determine the parameters that you think affecting the enzyme production (such as pH, temperature, incubation time...etc.). Then you can reduce the parameters by using statistical designs like Box Benkhen which helps you to select more significant parameters. Then you have to do the experiments which the statistical program (like Design Expert, Minitab...etc) gives you exactly in the same order. At the last program will estimate the optimum values for your response (like maximum enzyme activity). That's all.
You can find more information below:
Regards,
Selim
Dear Ahmed,
I can recommend the following publication on medium optimization for fructofuranosidase production:
https://www.ncbi.nlm.nih.gov/pubmed/20502893
Best tool for DoE is MODDE from Umetrics (today mks). They provide a 30 days demo version:
https://mksdataanalytics.com/downloads/modde
If you are familiar with the mathematics behind DoE (factor coding, ANOVA, F-tests, model prediction, etc.) you can implement it in Excel or R.
https://www.youtube.com/watch?v=7DanDxiXQAk
https://www.crcpress.com/Design-and-Analysis-of-Experiments-with-R/Lawson/p/book/9781439868133
Give it a try.
Best, Michael
You can try 30 days free trial from JMP software too https://www.jmp.com/en_gb/offers/free-trial.html
You can try 30 days free trial from MINITAB software https://www.minitab.com/es-mx/
Dear Ahmed Sobhy ,
First, you have to identify all the influential parameters (such as agitation, temperature, incubation time, substrate concentration, pH etc), having an effect on enzyme production. You can choose the most influential parameters based on Taguchi analysis or Plackett-Burman analysis. Thereafter, you can create your DOE (design of experiments) using selected parameters. And, further experimental results should be analysed by RSM (Response surface methodology). The statistical analysis can be performed by Design expert or Minitab. You can try the free version/student version.
Please refer these for your study
Thank you
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