Hi, I have a problem with my mouse Adipose MSC primary culture?

I am working on mouse adipose mesanchymal stem cells. After removing the mouse peritoneal fat and extracting, I do the primary culture, I used DMEM F12 medium with 15% FBS, but with this, the...

13 February 2021 9,838 1 View

How to estimate total electron content using GPS data (with MATLAB)?

06 January 2021 1,110 2 View

How can I find the area of contour with magnitude less than specific number?

Hello everyone I have results from fluent and I want to determine area with magnitude less than specific number for example for velocity or TKE. and also how can I determine Max and min diameter...

02 November 2020 5,863 3 View

Why residuals stay at the same range in vof model in Fluent and it doesn't converge?

hi I'm modeling a 3d channel with vof , k-epsilon RNG with non-equilibrium wall functions , for bc I have velocity inlet, pressure outlet and for top of the domain I have used pressure outlet...

21 August 2020 5,926 7 View

I am confused about the editor's request (I had submitted a manuscript and then major revision was requested). Can anybody help me?

I had submitted a manuscript to a journal. After a while, they requested major revisions. The end of their request, they mentioned that: "You will be unable to make your revisions on the...

09 August 2020 5,436 7 View

Is it possible to extract RNA and protein from 96-well plates?

I am doing a knockdown test in 96 well plates and then I need to extract RNA and protein from my cells. I'm not sure that the number of cells in 96 well plates is enough to these extractions....

12 June 2019 5,459 3 View

In SDS-PAGE , how possible to use freez-dry samples for loading in gel electrophoresis?

I freeze fungal mycelia of mushroom. In SDS-PAGE of total protein ,If I freeze mycelium I can used this sample? How should I prepare mycelium for electrophoresis? How should I make it for...

01 October 2018 7,971 5 View

What is the extinction coefficient of pyrogallol at 430nm for calculation of PPO(polyphenol oxidase) activity?

I performed the polyphenol oxidase activity assay from certain mycelia extracts using pyrogallol as susbtrate. I have the absorbance values but am facing the problem in determining the enzyme...

05 August 2018 172 1 View

Many thanks for all your help?

This question has been deleted.

30 March 2018 7,374 5 View

Is there any software to determine the number of codewords with fixed GC-content in DNA codes?

If so where can I find it?

03 January 2014 9,116 8 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to search for crystal structure for material with atomic position labelled?

I am using the crystallographic open database and materials project to search for cif file but the exact structure is not mentioned or how can I deduce that the given structure and atomic position...

23 February 2021 6,723 2 View

How to generate AA6061 alloy or any aluminium alloy crystal in material studio?

I need to carry out molecular dynamics simulation on Aluminium alloy. For this i need the crystal structure that which could be further replicated to form a structure.

22 February 2021 1,410 1 View

Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View

How to resolve Gaussian error termination " Error termination via Lnk1e in F:\G16W\l103.exe" for TS(QST3)?

When I run the job for TS(QST3) in gaseous phase, it was completed without any error. But when I add water in CPCM model it terminated with an error: Inconsistency: ModMin= 2 Eigenvalue=...

21 February 2021 3,198 2 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

How to calculate the Atomic Polar Tensor using DFT ?

Dear friends, I am a beginner at DFT calculation. I have done a structure optimization using DFT- B3LYP/6-31G*, Nevertheless, I don't know How to calculate the Atomic Polar Tensor (APT) in...

17 February 2021 9,082 3 View