I have got 22-25 colonies on my after transforming the ligation in DH5aplha. Should I screen all the colonies?

I have screened 10 colonies out of 22. But neither get insert release nor the PCR amplification for the desired gene. What should I do, should I further screen the colonies?

02 December 2020 3,225 3 View

Which bacteria can grow on 10x PBS buffer?

I have prepared PBS buffer and components for M9 medium and I store it in freeze, I do not use it if it is more than 2 weeks old. From time to time I check the sterility of these solutions and...

04 November 2020 9,427 6 View

Which model can be used to predict on below data?

I want to predict valuation using verbal SAS but there exists a strong nonlinearity between the two variables which makes unfit to use linear regression. Please see the attached scatter plot image...

05 August 2020 1,602 3 View

Which is the best model to predict student marks based on entrance exam result?

Hi, I have a dataset that contains student data that are nested based on different schools where more that one student belongs to a particular school. student_id school_id non_verbal...

29 July 2020 9,825 18 View

One step RT-PCR enzyme optimisation, can anyone help?

Helping others is the way we help help ourselves! In this case literally!! I work at a biotech company called Genaxxon Bioscience GmbH in Germany. We have an amazing one step RT-PCR enzyme which...

13 July 2020 7,395 2 View

Do Acetonitrile type (gradient grade or non gradient grade) affects the HPLC results?

Trying to develop the HILIC-RID method for sugar analyte but getting very different results between two-run of the same sample. Tried 60 minute washing between two-run. The mobile phase is 75:25...

11 June 2020 8,385 3 View

Why Pseudomonas fluorescens ATCC13525 does not grow on acetate?

I am trying to grow Pseudomonas fluorescens ATCC13525 on different carbon source except glucose in M9 medium but i cannot see them growing on acetate. I am not able to find more information about...

20 February 2020 2,469 6 View

Which funding agency in India will grant traveling allowance for international conference in finance ??

I get an opportunity to present my paper in international conference on June 2020. I am research scholar in management (finance department).Kindly let me know which funding agency will grant...

07 January 2020 7,040 1 View

Is raman peak at 515cm-1 normal for silicon wafer with 532 laser and 600 grating?

We installed new grating to your Renishaw inVia Raman microsscope. While routine calibration of the system, we observed the Si raman peak at 515cm-1 for 532 lase 600 grating and 519.9 cm-1 for 532...

02 December 2019 5,582 3 View

Why does my freshly thawed and seeded HeLa cells look different than usual?

I had stored HeLa cells in -80C freezer for over a month. When I thawed and seeded the cells today, I noticed that the cells looked significantly different than usual cells when floating in media....

25 November 2019 4,352 6 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View