I am working on the isolation of salicylates fractions and analgesic, anti-inflammatory activity. kind guide on how to isolate the fraction using the silica column. thank you.
Chromatography is a technique by which one can separate organic compounds from mixture. Use a glass column, and fill silica in it upto the level you need. Then make a slurry of organic compounds using organic solvent and silica. After compleat evaporation of the solvent, pour the slurry in the mentioned column. Then pour the solvent in it accordingly .
First, run a TLC plates; this lets you know what solvent systems will work. Are you interested in the salicylate esters, or salicylic acid? The free acid will probably need an acidic modifier added to the solvents to maintain a good peak shape. The free acid will probably need dichloromethane/methanol solvent systems, while the esters will probably elute with hexane/ethyl acetate.
If you have access to reverse phase, this works well for these compounds and may even resolve the esters better than silica.
If you are extracting with methyl acetate, the compounds should run with hexane/ethyl acetate. You might need dichloromethane/methanol since some more polar compounds can be extracted with this ester (or ethyl acetate).
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