It is well established that higher dose of nitrogen application reduces orobanche germination. But the mechanism was not clear. If any body have clear understanding on this, please kindly share it with me.
Application of Excessive Nitrogenous fertilizers especially urea or ammonical form of nitrogen during conditioning and germinating phases has been reported to reduce the germination, radicle length and weed proliferation. As mentioned earlier Low or absence of glutamine synthase (GS) activity in this weed may contribute to sensitivity to N-fertilization. However, the knowledge about the N-inhibitory mechanism of this weed in relation to their host which still continues to be elusive. But some of the papers I am enclosing for your reference
Broadcasting nitrogen fertilizers in ammonical form excessively at early growth stages increases nitrogen loss in the form of ammonia gas (NH3) that is toxic to seedlings.
The Orobanche seed weights ranging from about 0.1 g per 1000 seeds to about 3.0 g per 1000 seed,. Upon germination seed develops a small radicle which penetrates a fine rootlet of the host and becomes firmly connected with it at that time the application of high rate of applied urea may produce uric acid or ammonical acid and damage the germinated radicles of seeds as well as stop the germination's
Instead of controlling Orobanche by N application, the practice being expensive, it is better to grow cotton in rotation in place of sorghum, to control this root parasite.