How extinction coefficient of a polymer is changed in presence of nano particle?

We have synthesized a fluorescent conjugated polymer. The optical density (OD) of the polymer increases in presence of nano particle. We have also taken the OD of the polymer and the nano particle...

10 November 2019 5,102 4 View

How can I measure the thickness of a spin coated thin film?

I have made a spin coated thin film of a fluorescent polymer. I want to measure the thickness of this film. In which techniques or machines I can get it? Thanks in advance.

10 November 2019 669 7 View

How the fluorescence lifetime is changed with the conformational change of a fluorophore ?

If the conformation of a fluorophore is changed from cis to trans or trans to cis then how its fluorescence lifetime will vary ?

04 May 2019 8,057 3 View

How can I calculate the half reduction/oxidation potential from a CV graph?

Half reduction /oxidation potential

11 December 2018 8,982 0 View

From SEM picture, how do you identify the difference between co-crystallization and inclusion?

How can be determined from the SEM data of an inclusion (Host-Guest) complex that the guest is included into the host or a co-crystallization of host and guest?

09 October 2017 7,103 1 View

How red shift and blue shift depends on the polarity of solvent?

31 December 2016 9,962 13 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

Interpretation of a fluorescence Emission spectrum ?

Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...

20 February 2021 1,291 3 View

Good day sir/ma. Please, where can I have access to equipment for conducting XRF, XRD and SEM analysis?

I'm using blast furnace slag for stabilizing subgrade soil.

17 February 2021 7,333 3 View

Fluo 3-AM in pancreatic beta-cells?

Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...

16 February 2021 453 2 View