What markers could be tested to confirm NGF-mediated PC-12 Adh differentiation?

Greetings, While PC-12 cells are commonly differentiated using nerve growth factor (NGF), I am going to be using PC-12 Adh (the adherent version of PC-12 cells) for a series of experiments...

04 May 2017 7,052 9 View

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10 November 2016 4,615 3 View

How are the levels of global protein expression affected in the G0 phase of the cell cycle?

I wonder if cells slow down their protein expression and keep reusing the ones they have already made since they're not going through different phases and so the need to make/degrade proteins is...

02 March 2016 3,266 2 View

Would either vitamin A or D in skim milk have any adverse effects on the western blots?

Hello, I only had access to skim milk supplemented with vitamins A and D to do my western blotting. Do you have any comments on the possible effect(s) the vitamins might have on the results? Maybe...

01 February 2016 2,376 5 View

What do you think these extra WB bands are?

Hello! I did a transient transfection with a FLAG-tagged construst (FLAG-protein) and used the empty vector which has 3XFLAG (EV) as well as no plasmid at all (Mock), as my controls. The darkest...

11 December 2014 2,904 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View