Shahin Khazai

6 Questions 15 Answers 0 Followers

Questions related from Shahin Khazai

How does one go about detecting/identifying bacteriophages in mixed samples?

Hi! How are phages detected/identified in mixed samples such as soil, water, or even tissues?

09 September 2017 4,557 5 View

What markers could be tested to confirm NGF-mediated PC-12 Adh differentiation?

Greetings, While PC-12 cells are commonly differentiated using nerve growth factor (NGF), I am going to be using PC-12 Adh (the adherent version of PC-12 cells) for a series of experiments...

05 May 2017 7,177 9 View

What are the negative effects of long-term storage of animal cells at -80°C as opposed to in liquid nitrogen vapour phase or liquid nitrogen itself?

Hello! I know cell viability is the main concern when animal cell cultures are stored at -80°C for long, but what other negative effects do you know of? Any information at the cellular as well as...

11 November 2016 4,690 3 View

How are the levels of global protein expression affected in the G0 phase of the cell cycle?

I wonder if cells slow down their protein expression and keep reusing the ones they have already made since they're not going through different phases and so the need to make/degrade proteins is...

03 March 2016 3,349 2 View

Would either vitamin A or D in skim milk have any adverse effects on the western blots?

Hello, I only had access to skim milk supplemented with vitamins A and D to do my western blotting. Do you have any comments on the possible effect(s) the vitamins might have on the results? Maybe...

02 February 2016 2,435 5 View

What do you think these extra WB bands are?

Hello! I did a transient transfection with a FLAG-tagged construst (FLAG-protein) and used the empty vector which has 3XFLAG (EV) as well as no plasmid at all (Mock), as my controls. The darkest...

12 December 2014 2,989 2 View