I am pursing spectroscopic methods because I need either a non-destructive technique and/or high throughput,so I want to use the instrument of near infrared spectroscopy or fluorescence spectroscopy to measure PAHS in water.But I'm not sure whether it is feasible to do so.
Because of low solubility of PAHs in water, how to do the qualitative and quantitative analysis of the PAHS in water by using the instrument of near infrared spectroscopy or fluorescence spectroscopy? By using the instrument of near infrared spectroscopy or fluorescence spectroscopy,to measure PAHS in water is more difficult than in soil?
A purity of 99% of anthracene, fluoranthene, pyrene, phenanthrene PAHS powderertor need to be diluted to low concentrations.
Because of carcinogenic polycyclic aromatic hydrocarbons，if operator,wearing masks and gloves,do experiments of about PAHS in a fume hood,is it great possibility that the operator is more seriously contaminated by PAHs in laboratory environment than in everyday living environment ,such as smoking, air haze, etc.? Do anthracene, fluoranthene, pyrene and phenanthrene have more
carcinogenicity ?
Thanks very much
Are you pursing spectroscopic methods because you need either a non-destructive technique and/or high throughput? If that's not essential, then purge and trap GC would I think be the most typical method. Provided the concentrations are not too high, doing everything in a hood should keep operators safe.
I am pursing spectroscopic methods because I need either a non-destructive technique and/or high throughput,so I want to use the instrument of near infrared spectroscopy or fluorescence spectroscopy to measure PAHS in water.But I'm not sure whether it is feasible to do so.
thank you.
You could try using synchronous fluorescence spectroscopy. It is quick and inexpensive, so high throughput, but the drawback is that the level of quantification may be rather high for some compounds. I have used it for pyrene & phenanthrene. It might work for you if the waters you work with have one or a few PAHs with not too similar fluorescent characteristics, so if you are doing experiments in the laboratory and making e.g. exposure waters with pure compounds it should work for you (if the concentrations are not too low). But if you want to measure PAHs e.g. in natural waters you need GC-MS to be able to properly identify & quantify them.
How low will the concentrations be?
Standard methods in wastewater analyses will will probably be a good place to explore. But I think they are mostly going to be non-specific methods to do the high-throughput screening (i.e. broad UV absorption) and then something more specific, but destructive is used to confirm. EPA link:
http://nepis.epa.gov/Exe/ZyNET.exe/600012A6.TXT?ZyActionD=ZyDocument&Client=EPA&Index=1981+Thru+1985&Docs=&Query=&Time=&EndTime=&SearchMethod=1&TocRestrict=n&Toc=&TocEntry=&QField=&QFieldYear=&QFieldMonth=&QFieldDay=&IntQFieldOp=0&ExtQFieldOp=0&XmlQuery=&File=D%3A\zyfiles\Index%20Data\81thru85\Txt\00000014\600012A6.txt&User=ANONYMOUS&Password=anonymous&SortMethod=h|-&MaximumDocuments=1&FuzzyDegree=0&ImageQuality=r75g8/r75g8/x150y150g16/i425&Display=p|f&DefSeekPage=x&SearchBack=ZyActionL&Back=ZyActionS&BackDesc=Results%20page&MaximumPages=1&ZyEntry=1&SeekPage=x&ZyPURL
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