About loading of Doxorobicin on MOF (drug delivery)?

Dear Researcher Respectfully, I am working on drug delivery. I synthesized Zn-MOF. I am going to load Doxorobicin on it and the study the derivery behaviour. I tried several times to load. but I...

07 February 2021 748 2 View

How can I represent a 2-parameters function in series type?

I want to represent a function like f(x,y)=sin(1/(x+y)) about [x,y]=[0,0] in series type. how can i do it in matlab or maple?

10 January 2021 8,389 5 View

Can you recommend a protocol for immunohistochemistry of mouse brain sections using anti-MAP2?

Current protocol does not give clear images.

04 January 2021 1,614 3 View

Challenges in growing deep learning model?

I implemented an image classifier and object detection model. I added a new class every day to my model and the data set growing too. I wanted to ask if anyone has the same experience? at now it...

03 January 2021 4,652 5 View

Anyone knows how to extract RNA from samples with high starch content (wheat) by using RNeasy Plant Minikit?

I am using 74904 to extract RNA from wheat seed and I have tried both of the lysis buffer. As it is mentioned it protocol, buffer RLT is the lysis buffer of choice but it may cause...

14 October 2020 9,409 7 View

What causes different microstructure in a sample?

I have synthesized high entropy oxide with (Ni Zn Cu Mn)1-x Cox composition. At x=0.05 the microstructure became rod shape and the SEM picture of the other part of that sample is different. And...

12 October 2020 5,576 5 View

How to increase zebrafish survival rate after spinal cord injury ?

Hi everyone I'm having trouble with the high mortality rate of my zebrafish after surgery. ( the surgery is done based on this article: Becker, C. G., Lieberoth, B. C., Morellini, F., Feldner,...

13 September 2020 6,781 1 View

How long does it take for MCF-7 cancer cells to adhere to the microfluidic surface? Is 24 hours enough?

Hello everyone. We have cultured MCF-7 cancer cells in microfluidics several times and incubated them for 24 hours, but as the nanomaterial passed through the cells, the cells moved and no cell...

05 September 2020 7,292 3 View

Antibiotic screening of drug resistance cancer cell line with MDR pumps?

Hi. during our study, we faced with a problem in the screening of EPG RDB 257 cell line which is a resistant cell line due to the expression of efflux pumps such as MDR 1 which actively excrete...

27 August 2020 9,192 1 View

How to work with IQF device with the highest efficiency?

Hello I work with the IQF machine My problem is that when wet vegetables enter the machine, the efficiency of the machine decreases. What can be done to remove moisture from vegetables without...

01 August 2020 8,179 1 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

There are prominent crystal formation in my cell culture dish, what are the possible causes and how to get rid of them?

17 December 2020 3,093 4 View

Do ammonium glutamate considered as necessity in salt sloution for Ecoli-based Cell free protein synthesis platform ?

Hello, I am constructing an E-coli based Cell free protein system (CFPS) reagent, but I can't find ammonium glutamate from the local suppliers or from MP biochemical local disruptors. Can I...

13 August 2020 3,691 2 View

Why are my ARPE-19 cells exhibiting this morphology?

Hello! I am culturing ARPE-19 cells (passage 25) in DMEM + Sodium Pyruvate, Glutamine, 1% FBS and observing this phenotype in my cultures. These have been in culture for about 2 months and are...

12 May 2020 8,499 1 View

Is there literature on pre-steady state "lag" for oxidase enzymes?

Greetings, I'm looking for literature related to a topic I want to write a manuscript on. I'm working with an glycine oxidase enzyme and noticed when I monitor the rate via spectrophotometry and a...

31 March 2020 3,917 2 View

Can free glutamate ions turn dangerous?

can a non-human virus step on them, using those unbound particles, easier to conquest, to invade the host ? Glutamate only taste when unbound....

17 February 2020 5,423 10 View

Alternative Media for Expi293s (Currently Using F17)?

Does anyone know of a more affordable alternative to F17 media for 293Expis? We currently supplement with L-glutamine and Pluronic F-68.

10 February 2020 637 1 View

What clinical evidence is there for antiglucocorticoids, NMDA antagonists, or calcium channel blockers in the treatment of alcohol withdrawal?

I have seen studies citing effects of antiglucorticoids for example in mitigating cortisol effects and NMDA antagonists in managing glutamate effects in the brain during alcohol withdrawal. Is...

11 December 2019 7,582 3 View

How to eliminate the warning of 'position not found when parsing line' during DAMASK simulation with ABAQUS/standard?

Hi, I am trying to use the ABAQUS/standard solver to simulate the single crystal deformation behaviour with DAMASK framework. The materials is BCC ferrite with hooke elasticity and phenopowerlaw...

10 December 2019 4,667 1 View