I am measuring by qRT-PCR hepatic SCD-1 expression and I observed that SCD-1 decreases in diet-induced obese mouse livers, which is in line with the leptin-increased signaling in these livers. However, it has been shown that SREBP1c could be increasing SCD-1 expression. In other set of samples, short-term refeeding (it supposes to incrase SREBP1c-mediated upregulation of lipogenic genes) could not increase hepatic SCD-1 expression. Although it has been reported that SCD-1 is a direct target of SREBP1c in the liver, I think that some data are contradictory and SREBP1c may not be directly controlling insulin-mediated SCD-1 expression. Has anyone have any reference to support any of this contrary observations?