Just as the stated above, I just wondering how to relate between the result I obtained from the analysis of UV-Vis with the result from ftir for the analysis of plant extract.
I raised this question because a colleague told me that UV-Vis result is like an additional result to support the one you got from ftir.
It is difficult to correlate between the two methods. FTIR spectroscopy indicates the different frequency bands in the sample while UV-Vis identifies different compounds having a maximum absorption maxima.
Please understand that UV-VIS spectroscopy is used mainly as a quantitative analytical technique. UV-VIS spectrum cannot give much qualitative information because many compounds give a very similar spectra in UV-VIS. At the same time it is an excellent quantitative technique. FTIR, on the other hand, can provide valuable qualitative information. It can identify the probable functional groups in a given compound, which in turn will enable you to have valuable information regarding the identification of the compound. Generally speaking, if you have two different chemical compounds, A & B, both may provide more or less identical UV spectra, but both will provide totally different IR spectra. Hence, it will be difficult to differentiate two different compounds based on UV spectra, but it can be done easily by IR spectra.
Fundamentally, both FTIR and UV-VIS are methods that perturb your sample with energy (different wavelength range for each method). This energy probes different molecular interactions. IR (lower energy than UV-VIS) is useful for promoting different vibrational transitions where UV-VIS gets into the electronic structure of molecules. Both provide information about the sample.
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