Should I use smaller size (1000-3500 Da) or bigger size (12k-14kDa)? What are the other basis besides diffusion rate? I am conducting a research for inhibition of glucose diffusion using plant extracts.
The size of dialysis cutoff depends on the aim of your study. If you want glucose to diffuse through then choose a larger size than the size of the molecules you want to get past the barrier and smaller than any molecules you want to keep inside. So if you want to trap glucose in a larger molecule then make sure your cutoff is small enough to keep that inside. SO you may be need to know the size of your plant extract active components
Thanks!
Most of the articles I read used larger MWCO (12k-14kDa). If I want to trap glucose in a larger molecule, is it better to use atleast 200 MWCO or would you recommend using 1000-2000 MWCO? I wonder if the availability of smaller dialysis membranes at those times is one factor. I will use crude fractions of plant extracts so the size of the active components varies.
It really depends on the difference in mol wt of the key molecules. The low MWCO dialysis tubing are usually more expensive and not needed if you are trying to keep in ony larger molecules so choose one with MWCO less than the smallest larger molecule you want to keep in.
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