How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

How can I calculate convective heat transfer coefficient from turbulent boundary layer properties?

I know many textbooks include empirical relations between Nu, Re_x, and Pr inside a turbulent boundary layer on a flat wall. However, I want to directly calculate h from known boundary layer...

19 January 2021 1,294 3 View

ANSYS APDL tabular entry related?

Hello I want to add thermal convection load on specific area of concrete volume. it asks for film coefficient which is a constant value but the bulk temperature has no. of values that i have as...

18 January 2021 6,627 1 View

Any suggestions on 50um cryosection immunostaining (growth plate) ?

Hi everyone I am working on immunofluorescence of cryosection of murine growth plates. The Ab worked well on 8um cryosection. But when I stained 50um section, I found the fluorescent signals...

03 January 2021 9,531 3 View

Should I normalise data when measuring fluorescence intensity ?

I'm measuring fluorescence intensity of the product in an enzyme reaction in 37 celsius degree. I set a kinetic measurement in 1 hour. The fluorescence intensity of the BLANK ( contains only...

03 January 2021 5,554 5 View

Does anyone know any article on free convection heat transfer from an oblate with uniform heat flux?

Does anyone know any article on free convection heat transfer from an oblate spheroid with uniform heat flux? All articles I've found are about isothermal oblates.

14 December 2020 1,163 4 View

Is it better to normalize Nile red absorbance to Bradford assay or to cell number?

I stained the fat droplet inside the cell using Nile red and read the absorbance by a microplate reader. Which better to do, normalize the Nile red absorbance to Bradford assay or to cell...

12 December 2020 8,036 2 View

Assay with absorbance/fluorescence readout?

I'm setting up a liquid handling robot and need a simple assay to test it. Ideally I would like to mix 3-4 reagents and have a absorbance/fluorescence readout on a plate reader. It's also possible...

11 November 2020 7,645 3 View

Why can't my energy be delivered?

Hello, the software I use is ANSYS Fluent. The subject of my research is the analysis of temperature distribution in the oven. At present, I have a very simple model to test, just how to try the...

18 October 2020 9,531 6 View