How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Is there a mathematical model that describes the composting process?

These models can be of the different stages of composting, either from the thermophilic or mesophilic stage. Thanks

22 June 2020 3,462 6 View

Thermophilic gram- negative bacteria and spore forming which one ? name the bacteria or genus of bacteria ?

more information about thermophilic bacteria

11 August 2019 6,750 1 View

Is there possible if thermophilic bacteria have gram negative and forming spore?

I have isolate bacteria from hot spring. Based on Gram staining, the isolates were found gram-negative and microscopic observation revealed spore-forming rod-shaped bacterium. I'm looking for...

07 August 2019 4,074 4 View

What will be the optimum organic loading rate (OLR) for a two stage Anaerobic biodigester?

thermophilic followed by mesophilic set up. Indvidual optimum OLR for thermo and meso. As per literature review, Thermophilic olr is 5-8kg/m3/day and for mesophilic it is 3-5 kg vss/l /day

19 May 2019 3,335 8 View

How can be thermophilic enzymes denatured?

I need to stop the activity of thermophilic xylanase enzyme after enzyme hydrolysis, but it is not denaturated at 100oC or alkali pH (9) and acidic pH might damage the end product. I don't want to...

28 April 2019 604 9 View

How can be thermophilic enzymes denatured?

I need to stop the activity of thermophilic xylanase enzyme after enzyme hydrolysis, but it is not denaturated at 100oC or alkali pH (9) and acidic pH might damage the end product. I don't want to...

03 April 2019 9,203 11 View

Can anybody help me with the protocol on how to detect protease activity in a gel containing Casein substrate?

I have just cloned and got a soluble expression(50%) of a thermophilic serine protease and did the heat treatment for purification at 70 and 80 degree with over 90% purity, and also have a...

15 March 2019 9,330 3 View

Can anybody help me with the protocol on how to detect protease activity in a gel containing Casein substrate?

I have just cloned and got a soluble expression(50%) of a thermophilic serine protease and did the heat treatment for purification at 70 and 80 degree with over 90% purity, and also have a...

02 March 2019 5,568 3 View

Why does my freeze-dried culture of Carboxydothermus hydrogenoformans (DSM6008) not revive?

I have tried to revive the above organism from a freeze dried culture provided by DSMZ. As is usually the practise, instead of utilizing the entire culture at one go, we use sterile loops to...

01 February 2019 7,970 12 View

Can a freeze dried culture survive above-optimal pH for some time during revival?

I was trying to revive a freeze-dried culture of thermophilic carboxydotrophs, but accidentally the pH of the culture in which I inoculated the freeze-dried culture was much above the optimum for...

11 December 2018 8,548 0 View