Hello, I've been using Accutase recently to detach my adherent cells. I was previously using trypsin-EDTA. According to the manufacturer's indications, Accutase can be stored long-term at -20 and short-term at 2-8.

When at 2-8, do you store the whole bottle or you create aliquots that can be readily used?

Also, do you thaw at 4 or thaw in a 37 degree water bath?

Thank you.