I want to evaluate the effect of different treatments on the composition of microbial community. The treatments consist in different crop rotations and I have two repetitions (communities) per treatment. Every community is characterized as number of sequence per each taxa detected. To complicate the analyses there are some soil parameters (pH, P, N..) that are not uniform even among the repetitions. Is there any kind of analysis to split the influence of environmental factors from the influence of my treatments?
Hi,
Your question: How to split the environmental factors from the influence of agricultural treatment on microbial communities?
Kindly see the following:
http://aem.asm.org/content/64/1/238.full
https://www.researchgate.net/publication/222419547_Factors_affecting_soil_microbial_community_structure_in_tomato_cropping_systems
Article Factors affecting soil microbial community structure in toma...
The simplest way to separate the environmental factor from agricultural treatments in your experiments is to treat time (year, season, crop cycle, whatever applies) as a factor in a multifactorial ANOVA along with your agronomic treatments. The relative effect of the "environmental factor" on each of the variables you wish to examine may be deduced as the percentage fraction of the Mean Square Error attributed to this factor in your ANOVA relative to the total MSE (for this variable) that accounts for all factors, interactions and the experimental error.
Some environnmental factors influence growth and production of antimicrobiens metabolites in soil: temperature, source of carbone, pH, pourcentage of inoculum.
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