Dissolve 12.5g of Nutrient Broth No.2 in 475ml of distilled water and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C or below. Aseptically add 25ml of Lysed Horse Blood SR0048, 1 vial of Preston Campylobacter Selective Supplement SR0117 or SR0204 and 1 vial of Campylobacter Growth Supplement SR0232. Aseptically dispense 5ml volumes in sterile small screw-capped bottles. The Selective Enrichment Broth may be stored for up to 7 days at 2-8°C. The broth can be prepared by mixing 1.88g vegetable consommé powdered broth of which glutamic acid has been removed with 500 mL room-temperature distilled water (plain broth) or a similar volume of a 0.4% (0.021 M) monosodium glutamate (L-glutamic acid monosodium salt monohydrate, Sigma-Aldrich, St. Louis, MO) solution (savory broth)