What is the nature of the undesired current response peak in PUND technique for ferroelectric characterization right before U and D pulses ?

Hello, Recently I used the Positive Up Negative Down technique to calculate the polarization of PZT. After the experiment, I observed two unwanted current response peak which appears right before...

04 October 2020 6,736 3 View

What is the difference between CB-SEM and PLS-SEM?

Dear all, I am searching about how to conducting SEM analysis. Could you please clarify the difference and conditions to use for each model?

05 June 2020 6,496 4 View

Convert .py file to .exe using pyinstaller .exe just blink. How to fix it?

I am new to python and am making a web scraping program to automatic collect information about scholarship for me and I want to share it with my friends. It has few dependencies. It run...

09 April 2019 7,524 3 View

How to determine PM flux linkage, Ld, Lq corresponding to rotor position?

In these attached papers, the authors said that it can be used frozen permeability (FP) to determine PM flux linkage, Ld and Lq corresponding to rotor position, Fig. 11 and 12 of the first paper,...

13 December 2018 6,855 2 View

How can I find out the avaiable Phasor Measurement Unit dataset?

Dear scholars, Currently our group is working on the machine learning models of synchronized phasor measurement unit? We knew that because of the security issue, these PMU dataset are ussually...

01 February 2018 251 5 View

Does cell death affect the genomic DNA for its ability to be amplified by PCR?

I am trying to amplify a DNA fragment of about 500 bp. The control which is the DNA extracted from the last cell sample. This DNA sample allows me to amplify the region, which proves that the...

06 December 2017 2,554 3 View

Can FLAC3D perform a coupled Thermal-Hydraulic-Mechanical simulation?

FLAC3d can be employed to perform Hydraulic-Mechanic simulation of soil, rock or something else but how about Thermal - Hydraulic - Mechanic simulation?

02 August 2017 3,780 1 View

Detect aninline

Hi. I want to use Thin-layer chromatography to determine aniline . But i can not find how i can do it (method, Solvent system, Composition)? How to detect the aniline line?

14 June 2017 5,889 3 View

What range of Coefficient of Variation (CV) we can accept when performing a field-experiment?

02 December 2016 1,399 14 View

From statistical point of view, is the usage of composite variable recommended in SEM AMOS?

Is considering to impute data to create composite variable using regression imputation in AMOS. With that, structural model would be free from observed items.

23 June 2016 8,776 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

What is the composition of the solid and broth agar to grow Streptomyces from freezed sample? what are extraction steps of products from broth agar?

Please what are the composition of the solid agar could I use to grow the bacteria? what are the composition of the broth agar could I use to grow the bacteria and how to extract the natural...

26 January 2021 5,006 1 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

How to reduce or increase CFU/ml value ?

I collected the water sample from pond and 1 ml of pond sample diluted with 9ml with water and 100 microliter was cultured into the MacConkey agar plates .Finally,80 colonies were formed in the...

24 January 2021 1,925 3 View