What could be the possible reason for lack of Mtb protein expression cloned in pET28a even after induction ?

12 December 2020 2,584 8 View

Why the IT services companies (based in India) are mainly patenting only at the USPTO?

Please suggest some papers/reports on the changes in the policy with respect to patenting of computer software in India. Does it have any implications as to why the IT services companies (mostly...

12 November 2020 8,351 1 View

Can I combine data from two separate fluorometeic experiments?

I performed a fluorometric experiment with two different cell lines. I had four conditions per experiment, and four "sub-conditions" per condition, wherein I add three different chemicals (the...

08 November 2020 5,113 3 View

Can i use surfactant C-TAB in extraction and detection of the aminaphthalene sulfonates by HPLC technique?

In the detection of Aminonaphthalene sulfonates the quaternary ammonium salts like Tetrabutyl ammonium bromide ,Triethyl ammonium acetates are used so the C-TAB being an quarternary ammonium...

21 September 2020 9,636 1 View

Please suggest some good quality and free journal with very short response time for MEMS, piezoelectric energy harvester research?

Please suggest some good quality and free journal with very short response time for MEMS, piezoelectric energy harvester research?

27 August 2020 994 4 View

What is the critical chloride concentration for concrete made of GGBS and for concrete made with Silica fume ? Is it 0.5% ?

Ccrit or Cth values for concrete made with different mineral admixtures

21 July 2020 7,301 2 View

Objective function for Pulp library in python?

How do we define objective functions while doing optimization using Pulp in Python. We have defined Prediction models separately. Next step is to bring objective functions from prediction models...

06 July 2020 9,256 3 View

How i can dry Metal Nano crystals ?

freeze-drying is recommended for nanocrystals drying but other than this technique direct heat is useful to Metal Nano crytals

17 June 2020 1,238 3 View

I need to predict the time when a physical test on a machine will fail. Which time series forecast/ machine learning method should I use?

There are 2 columns: time(in seconds), amplitude of wave. I need to predict when the test may fail and we can observe a crack on the machine based on the amplitude. I do not know the...

30 May 2020 7,594 13 View

IDT or Geneart, which is best for full lenght gene synthesis?

Did anyone got full lenght genes synthesized from both IDT and Geneart and which one will be the best in terms of pricing, post purchase support and delivery speed. Preferably in India but the...

14 May 2020 942 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View