In fluorescence studies the FL peaks are obtained with varies fluorescent intensity. Can anyone guide me on how to obtain the integrated fluorescent intensity?
Hi
You could integrate and find the area under the curve using the integration tools in various programs such as: origin, igor pro, sigmaplot. You could use functions in matlab. It appears that you can even use freeware like gnuplot (in python).
Here is the gnuplot code for integrating in a python environment:
https://www.physicsforums.com/threads/gnuplot-how-to-find-the-area-under-a-curve-integrate.382070/
Hope this helps
Hi
You could integrate and find the area under the curve using the integration tools in various programs such as: origin, igor pro, sigmaplot. You could use functions in matlab. It appears that you can even use freeware like gnuplot (in python).
Here is the gnuplot code for integrating in a python environment:
https://www.physicsforums.com/threads/gnuplot-how-to-find-the-area-under-a-curve-integrate.382070/
Hope this helps
Thank you too much Madhavan . I often use origin soft ware and I am familiar with that. however, I try with it too. Thankyou once again.
Before you integrate make sure that the emission spectrum is corrected for the instrument response.
Determine the area under the peak either by using a software or manually by yourself.
This needs to be done after background subtraction and after correction of differences in spectral sensitivity of the detector.
In real world, one does not deal with ideal detectors i.e., Detectors may not be equally sensitive at all wavelengths. Hence the difference in sensitivity at different wavelengths has to be corrected for.
if the peak widths are identical, then peak height alone should suffice. But if the peak widths are not identical then the area under the curve needs to be considered.
Area under the curve can be obtained by integration which can be performed using any data analysis and data processing software. Many software like Origin, Sigmaplot etc can do this. Or one can also do this by writing a small routine in C, C++, Matlab, Labview etc.
There are also some tools available freely on internet which can do this.
Please see this video , it show the procedure for area calculation using origin software
How to calculate area under the curve in origin
https://www.youtube.com/watch?v=HwuvXcxz9fg
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