I have been trying to make a 0.5M EDTA solution by dissolving EDTA disodium salt dihydrate in 500mL of purified water and adjusting the pH to ~8.0 so that the salt dissolves. To aid in the dissolution I have put the solution on a stir plate and heated it slightly. However, I need to bring the pH of the solution back down to 7.4 for my experimental design. The issue I am having is that I need to add a large volume of HCl to the solution to decrease the pH , which then decreases the concentration of my EDTA. Does anyone have any suggestions to get around this issue?
Hi, Tess
What is that extra volume and and concentration of HCl solution you add to adjust the pH? What has come to my mind is that you can try to substitute some volume of purified water by HCl. In this case the final volume will remain 500 mL. However, you can try stronger HCl solutions, so the required volume of it is much less than now. Anyways, acid concentration is a variable I'd recommend to play with first of all.
Hello,
I am adding around 200mL of 0.5M HCl in order to bring the pH down. However, I have not yet tried moving to a stronger acid concentration. Thank you very much for your advice, I will play around with the acid concentration today!
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