I have performed an experiment to generate three standard curves for two reference (housekeeping) genes and one gene of interest using the Roche LightCycler480, and would like to know how to now (if possible) apply them to perform an Advance Relative Quantification analysis.

The LC480 manual suggests that this can be done, however appears only able to import one standard curve (that of the target gene) for the analysis. The output completely lacks any relative quantification data, and generates absolute quantification data under the "Target Name" tab only for the target gene (see image).

Is there a way to perform this analysis using the software or manually using the absolute quantification analysis output? I would really prefer to use the E-method as this accounts for numerous factors that can affect quantification, particularly differences in efficiencies across genes.