Kealan Pugsley

3 Questions 3 Answers 0 Followers

Questions related from Kealan Pugsley

Any recommendations/advice/protocols for the use of CRISPRa to upregulate a target gene in hiPSCs/hiPSC-derived neuronal cell lines?

I am looking to model how expression of a target gene from a state of dysfunction (i.e., knockout; CRISPR-Cas9 induced) to overexpression may influence aspects of neuronal functioning/morphology...

22 January 2023 9,513 2 View

How do you/can you apply external standard curves for Advanced Relative Quantification using the LC480 Software?

I have performed an experiment to generate three standard curves for two reference (housekeeping) genes and one gene of interest using the Roche LightCycler480, and would like to know how to now...

17 August 2021 150 0 View

How do you correctly apply No-RT controls in cDNA synthesis and qPCR?

I have cDNA samples extracted from 90+ individuals which I intend to utilise in a series of qPCR experiments to assess the influence of a specific SNP genotype/s on target gene/s expression. I...

09 July 2021 3,020 3 View