Kealan Pugsley

2 Questions 3 Answers 0 Followers

Questions related from Kealan Pugsley

Any recommendations/advice/protocols for the use of CRISPRa to upregulate a target gene in hiPSCs/hiPSC-derived neuronal cell lines?

I am looking to model how expression of a target gene from a state of dysfunction (i.e., knockout; CRISPR-Cas9 induced) to overexpression may influence aspects of neuronal functioning/morphology...

22 January 2023 9,466 2 View

How do you correctly apply No-RT controls in cDNA synthesis and qPCR?

I have cDNA samples extracted from 90+ individuals which I intend to utilise in a series of qPCR experiments to assess the influence of a specific SNP genotype/s on target gene/s expression. I...

09 July 2021 2,979 3 View