How do you amplify a low-gain signal in RNAscope multiplex FISH?

12 December 2017 1 5K Report

I am troubleshooting an RNAscope ISH multiplex fluorescence assay in PFA-fixed mouse brain. One of my probes works very well but the other signal is very light. Any suggestions for further amplification of the low-intensity signal and/or improved background blocking?

Jai Ghosh

I think the attached article might be of some help to you.

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