How do we calculate platelet distribution width in percentage from femto-litter. I have some data in fL and some in %. How to inter convert these two?

More Numan Majeed's questions See All

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

Connection between Matlab and visual studio?

i am working in transfer of images between two computer . I don't know how to make connection between Matlab and visual studio . i used a visual basic for programming my controller. Can anyone...

31 December 2020 6,708 4 View

What is the reason of suddenly increasing the concentration while evaluating the photocatalytic performance by RhB dye degradation?

I am doing a photocatalytic performance evaluation by RhB dye degradation. I am facing a problem which is: After achieving the absorption -desorption equilibrium, I checked the concentration and...

04 December 2020 3,922 20 View

NOVA 2.0 - Advanced Electrochemistry Software?

How can you help me to get a program NOVA 2.0 - Advanced Electrochemistry Software?

26 November 2020 3,873 4 View

I would like to ask what if I perform an extra CaCl2 treatment to make competent cells?

I would like to ask that I have tried to make competent cells by 0.1MgCl2 and 0.1mM of CaCl2 treatments. My question is, what if I perform a real quick washing of bacterial pallet with 0.1mM CaCl2...

07 October 2020 7,274 6 View

Is there anybody in US have a Prep chiral Column to separate a racemic compound ?

I am looking for sombody in US who use a chiral prep column to separate a synthesis racemic H-tetrazol compound? I will send him the synthetic materials and the successful separation conditions...

16 August 2020 4,356 2 View

What are the investigations that should be done to the patient suspected to be infected with corona virus( COVID -19) other than PCR?

What are the investigations that should be done to the patient suspected to be infected with coronavirus( COVID -19) other than PCR?

02 July 2020 8,746 7 View

How do you think that assessment for practical parts of any subject could be achieved by online means,and how far it would measure the student skill?

Due to covid-19 breakdown, most of education systems converted to whole online learning, and as the final semester for the current year is on the end, assessments must be done to measure if the...

29 May 2020 2,521 7 View

SDN Router Selection how to implement using python ?

algortihm 1 basic link selection input: txm matrix M, integer k output: MP =(M1 M2) where M1 is txk 1: compute the SVD of M: M= Udelta V T; 2: Apply QR with column pivoting to first k column...

16 May 2020 8,346 7 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Difficulty in debugging code to correct error - TypeError: '<' not supported ?

I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...

22 February 2021 5,254 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Antonio La Gioia

The formula to calculate PDW is: SD/MPV (in fL) and [SD/MPV]x100 (in %).

Example: PLT SD = 1,5fL; MPV = 9,6fL>>>PDW:(1.5/9,6)x100 = 15,6%

PDW:(1.5/9,6) : 0,156fL

Numan Majeed

Antonio La Gioia thanks a lot for the reply.

I have only values of

Platelet count

plateletcrit

MPV

PDW (in %)

platelet cell concentration

Platelet larger cell ratio

I need PDW in fL

Is it possible?

Dear Numan

I apologize for a mistake in the example of my previous reply that I beg you not to consider.

Both PDW and RDW (for RBC) have 2 different calculation mode (at least):

PDW (and RDW) CV (in %): 100x [CV/MPV (MCV)]. CV of cellular volumes is calculated from the respective histograms at the baseline

PDW (and RDW) SD (in fL): 100x [SD/MPV (MCV)]. SD of cellular volumes is calculated from the respective histograms at the level of 20% (the peak of the histogram is 100%).

So, missing the SD at 20% level, you can't calculate PDWin fL.

However, we must be aware that neither the MPV nor the PCT nor the PDW have any consolidated diagnostic relevance. All three measurements are dependent on the type of analyzer and are affected by pre-analytical situations such as anticoagulant and time spent in it.

Sean Ramrekha Harrynarine

I also have some PDW data in % and wish to convert to fL.

Babi Dutta

There is no way to convert them into each other unless you have a platelet width graph vs an absolute platelet count (expressed in %) graph from which you can calculate the PDW which is the distribution curve of platelets (fL) measured in 20% relative height with a total curve height of 100%, so if we have fL conversion is not possible until we have such a graph... I am also suffering from the same issues unfortunately I didn't find a way around it.. if anyone has a formula please share...