How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Does anybody know the ways of homogeneous ripening of Mung bean crop?

Some crops (Mung bean) are matured at different times i.e., harvested at several times that's why the labour cost of the farmer is high. So, could you please, is there any ways or chemicals are...

12 February 2021 294 4 View

How much could knowing the maturity level of an organization contribute to a given process?

The doubt is raised because there are several methodologies to estimate the degree of maturity of certain processes, such as computerization, communication, human resources, among others, and...

03 February 2021 4,753 7 View

How can solve/disperse silicon nanoparticles in water?

I want to add silicon nanoparticles to my hydrogel, but when I sonicated these particles with water to disperse them in water, they agglomerated on the water's surface. How can I disperse them in...

26 January 2021 213 4 View

Hi friends. Is it possible powder graphene disperse in organic solvent?

Dear friend I would like to ask you a favor. how can I reach to a solution of Graphene based in an organic solvent. and if it was possible, what the appropriate solvent would be? My current...

11 January 2021 9,163 3 View

How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for Metabarcoding?

I'm working on pollen DNA metabarcoding to identify the floral composition of honey. How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for the...

28 December 2020 9,132 1 View

What does this PZC data mean?

I measured different kinds of catalysts which were dispersed in DI water.I firstly added certain amount of HCl and then used NaOH to titrate and measure the pH value. What does this result mean?

27 December 2020 5,728 4 View

How to define custom boundary condition between particle and wall in comsol multiphysics?

I was trying to incorporate stick-slip boundary condition between particle and wall. COMSOL is having a stick and bounce model for that, but it is not suitable for my case. Is there a way I can...

23 December 2020 5,883 1 View

Is there any correlation between mature miRNAs and their precursors?

I have a series of RNA-seq datasets that their average spot length is about 90bp. (don't include small RNA reads like mature miRNAs). I'd like to do "Gene" and "precursor miRNAs" expression...

07 December 2020 7,744 3 View

Do the anchoring condition at NP-LC interface changes in any particular solvent (i.e. toluene, ethanol etc) or remains the same ?????

For absorbance and emission measurements of nanoparticles dispersed nematic liquid crystal we dissolve sample into a particular solvent (like toluene, ethanol etc). do anchoring condition at...

04 December 2020 1,938 1 View