HPLC: how to calculate violaxanthin area if it overalps with neoxanthin?

Does anybody know of, or have a method suitable for HPLC analysis that allows to separate violaxanthin from neoxanthin? I want to quantify the xanthophyll cycle (violaxanthin, antheraxanthin and...

21 January 2021 5,775 5 View

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07 December 2020 6,382 2 View

How does the His-Link Protein Purification Resin (Promega) perform?

We have always used Probond (Invitrogen) but Promega resin is cheaper. I was wondering how the Promega resin performs, to see if it is worth to change brands. Thank you!!

18 October 2020 4,351 3 View

How should I approach a Multilevel (2-1-1) Mediation Model with Categorical IV in SPSS or R?

Hi, In my study I have to test a mediation hypothesis in a multilevel (2-1-1) non-longitudinal model, with a categorical IV. I have access to R and SPSS, eventually to MPlus as well. As I...

30 January 2020 2,111 2 View

Can anyone help me with the detection of amyloid beta by western blot?

Can anyone help me with a working protocol for amyloid beta detection by western blot? I have extracted the whole proteins from the cells. For the Western blot I used a Precast gel 4-20% (in SDS...

25 November 2019 3,217 4 View

How to open pictures taken by using InCell2200 (file xdce) in ImageJ?

Hi everybody, I have problems in opening a .xdce file. The file is supposed to contain several series of images taken with the microscope InCell 2200. I tried to open the file by using either...

03 September 2019 4,096 2 View

Does the CellROX dye detect intracellular H2o2 or ROS in general?

Hi, I am looking for a dye to detect H2o2 in living cells that is not fluorecent in green because my cells are already GFP positives. Does anyone have any suggestion? I found the CellRox dye but I...

04 October 2018 4,770 3 View

How can i properly select the solvent to perform an extraction or to prepare a column?

I would like to attach a carbon triple bond to a benzaldehyde ring and to do that i am trying to perform a williamson reaction: https://en.wikipedia.org/wiki/Williamson_ether_synthesis. The two OH...

11 September 2018 4,206 3 View

Gene sequencing to check a SNP?

Hello, I have two cells lines: A375 and Mel-ST. This last one is an immortalized cell line from primary melanocytes. The BRAF gene should be mutated with V600E mutation in A375 cells but not in...

21 June 2018 8,561 6 View

How to cotransfect two plasmids?

I am trying to cotrasfect two plasmids into HEK293. One plasmid with cDNA of Clc1 gene (2967 bp) and second plasmid with cDNA of GFP (717 bp). Then I make patch clamp recording of chloride...

06 March 2018 3,017 3 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

Is there any rich, publicly available English literary corpus?

Hi everyone. I am looking for a rich corpus in English literature, a one which includes literary prose ( e.g., novels or short stories) . can anyone propose a good one? Best regards Fatemeh

01 March 2021 1,970 2 View

Is there a recitation score bank system for fair evaluation and assessment of student recitation?

In actual and virtual class, even if there is rubric for recitation, students may still fail to receive fair grades or scores. Others are active in recitation and others are not or others may fail...

26 February 2021 8,387 3 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

Must a control group to be in the same semester that a experimental group for a study in education?

Hi everyone, I'm researching about mobile learning in higher education. Due to the small number of the students in the semesters, I cannot have a experimental and control group together. I'm...

22 February 2021 3,653 11 View

Latent profile analysis based on two variables/sources of data?

Hello, for my master project, I would like to conduct a latent profile analysis about self-regulated learning skills. I would like to take two different measures into account when creating the...

21 February 2021 2,326 3 View

Where can I find raw quantitative datasets on ecosystem services?

I'm an Undergraduate student on the search for a research topic which has to involve any ecosystem service(s) and a response to environmental change. I'm looking for raw quantitative data sets (I...

19 February 2021 5,911 2 View

Which database is the most suitable for checking English bigrams frequency?

Which database is the most suitable for checking English bigrams frequency? We want to assess the frequency of different English graphemes. Most of the databases that we have checked seem to...

18 February 2021 4,565 3 View

How to validate image labeling quality within dataset?

Hello, it is about creating a dataset for semantic segmentation with three classes. The problem is that one class dominates with >90% and one class is

18 February 2021 4,254 5 View