In an in vitro assay, we test the adherence capacity of macrophages, but what cytokines, mediators, interleukin or integrins do macrophages use to adhere to polyethylene surfaces when stimulated by LPS?
Frida, thanks for answering.
I am looking for how this adhesion happens to find a way to explain why it was inhibited by a purified compound I'm studying.
Dear Raquel,
MQ like any other adherent cells can attach to the positive charge surfaces( polystyrene or polyethylene better to be coated by Laminin, Ploy L lysin, Collagen, Fibronectin and ...) by their surface glycoproteins which make the negative charge on their surface. Any factor that inhibit or block the function of this glycoproteins can cause the detachment of these cells.
Hope it would be of help!
Hi Raquel,
The answer to your question lies in work that was undertaken mostly in the late 1980's. The answer is complex and conditional.
I suggest that you find a library that still has paper journals and get hold of some of these now ancient texts. While you wait for the librarians to stumble up from the depths, you might find this is a useful read: Article Mediation of Biomaterial–Cell Interactions by Adsorbed Prote...
It doesn't answer all of your questions, but it will lead you in the right direction.
I cannot tell what the solution is without a lot more detail!
D
I agree with Frida , the cells will not use cytokine or chemokine to bind to polyethylene surface.The cells attach to plate by cell surface receptors and integrins .
Cell culture plastic were usually treated with a polymeric protein or polypeptide. For good cell attachment the hydrophobic polystyrene surface must be modified to a more hydrophilic surface. This allows cell attachment proteins (vitronectin and fibronectin) found in the serum containing culture medium to adhere and spread on the vessel bottom providing a better surface for cells to attach. However, recently Corning CellBIND culture surface was introduced in 2003 to improve cell attachment under difficult conditions, such as adapting cells to serum-free medium or recovery of frozen cells. They uses a higher energy microwave plasma to incorporate more oxygen onto the polystyrene surface rendering it more hydrophilic (wettable) while increasing the stability of the surface compared with traditional plasma or corona discharge treated surfaces.
I hope it helps you.
You can look at this article as well.
http://www.sigmaaldrich.com/technical-documents/articles/biofiles/evolution-of-cell.html