ligplot displays a 2D representation of a 3D model of protein-ligand complex. What validation did you want to do? 

Dear Matthew James Conroy Sir,

Thank you for your kind response.Actually Sir, I want to know how I save the file?because when I save the file it save as (drw) file format and it not open.So,kindly give your kind reply SIR

.drw files can be read by LigPlot

If you want to save a figure:

File > Write PS file

you should be able to open it with softwares that open pdf files.

Thank u Fillippo Prischi sir for your answer

Sir i also want to know one thing.

Discovery studio or ligplot which ligand interactions result more prefarable and why?

Because when i found interaction from both of software;they show different type of residue thats why i am little bit confused which result is more prefarable.

I'm not familiar with Discovery studio, but isn't it a 3D visualisation software? Try to read the manual of both softwares and try to see how they evaluate interacting residues, probably they have different settings.

There should not be differences between plot and structure, because, as Matthew James Conroy was saying, LigPlot is just a simplified 2D representation of a 3D model.

Dear Filippo Prischi sir,

Thanks a lot for your kind reply.Hope further you help me to solve different type of problems with your helping hands.