Where can I find the spike protein amino acid sequence of SARS COV-2 variant B.1.1.7?

I need the spike protein amino acid sequence of SARS COV-2 variant B.1.1.7 (also named as VUI 202012/1)

29 December 2020 2,409 3 View

Name the journals having highest impact factors in science and pharmaceutical science?

What is the name of the journal in science having highest impact factor? and What is the name of the journal having highest impact factor in pharmaceutical sciences?

03 June 2020 7,054 6 View

How much is the EELS plasmon peak reliable for calculating Fermi energy level for alloys and compounds?

In general the low loss electron energy loss peak contain the information about the electronic structure of materials. From free electron model (Drude Model) the plasmon energy is given by a...

26 May 2020 6,315 3 View

A vaccine effective in monkey against corona virus can be 100% effective in human?

An experimental COVID-19 vaccine protected monkeys from pneumonia and prompted a strong immune response in the animals. Vincent Munster at the National Institute of Allergy and Infectious...

15 May 2020 7,939 6 View

What is the difference between drug loading and Entrapmet Efficiency of drug?

During characterization of Nano-particles, i often get confused between these two terms. Please suggest.

23 April 2020 5,178 1 View

Zeta potential of nanoparticles apart from polymers depends on which factors?

Basically i prepared PLGA based nanoparticles by QbD approach. As Zeta potential is a Critical Quality Attributes(CQA) i have to explain the effect of CMAs and CPPs on CQA. Due to PLGA polymer,...

15 April 2020 5,035 5 View

Do we always need to have error degree of freedom greater than 12 to start our research ?

I have attended different universities during my study programme and for different conference and seminars, where I notice a critical number always suggested by almost every scientist/professors...

03 December 2019 420 3 View

Climate change is real. Is it really going to have some significant negative impacts on agricultural output ?

Climate change is real and over the years the weather parameters are changing, so the global hydrological cycle, atmospheric circulation, bio-geochemical cycles also changing. But to some extent...

25 November 2019 9,336 8 View

Can I transfect the ATDC5 cells with my gene of interest at 60% confluency and then allow the cells to undergo chondrogenesis?

I am interested in studying the role of a certain gene in the expression of Collagen type II and chondrogenesis per se in ATDC5 cells. But as per the papers for proper chondrogenesis to happen it...

25 August 2019 4,652 2 View

Would you like to provide me with questionnaires for character education?

I am writing a PhD thesis on caring ethics based on the thoughts of Nel Noddings. Since it is a philosophy theses, field study is not a requirement to be fulfilled. But as i am trying to look for...

03 February 2019 8,181 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View