I have deprotected acetyl group from protected galactose in a glycopeptide compound using NaOMe in MeOH, I want to inject this mixture to LCMS, is it OK to inject it directly without removing first?
Hello.
Which type of chromatography will you be making? Potential issues are not with the MS, but with the cinematographic system, although even these should be basically negligible. Still, for a definite answer, what is your separation principle and technique?
Hi Kindi,
I believe only catalytic amount of NaOMe was used. I think there are no major concerns injecting directly to the LCMS. Personally, I will be more comfortable removing the base first. You may wish to consider neutralising with amberlite cation exchange resin before injecting. This should get rid of the NaOMe without getting you worried about the integrity of your molecule.
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