Galega officinalis  Goatsrue is a member of the Fabaceae family contains a toxic alkaloid galegine. For its isolation plant parts are freeze-dried, ground, and analyzed with liquid chromatography/mass spectrometry. Galegine concentration is significantly different in plant tissues; reproductive tissues possess the highest levels of galegine (7 mg g−1), followed by leaf (4 mg g−1) and finally stem (1 mg g−1) tissues. Galegine concentration and pools varied over plant tissues and phenological growth stages. Galegine pools (dry weight by concentration) or the total amount of galegine per stalk are  lowest at the vegetative growth stage (2 mg stalk−1) and increase until reaching a maximum at the immature pod stage (91 mg stalk−1). The pools decrease nearly in half (48 mg stalk−1) by the mature seed stage. Like galegine pools, galegine concentration also reaches a maximum at the immature pod stage (4 mg g−1). The increased levels of galegine pools at immature pod stage corresponds with the time of meadow hay harvest, implying that goatsrue is potentially most toxic at the phenological stage. You can calculate by converting total green mass into dry mass and ratio  of plant plant having  galegine. It may 10mg/gm of dry matter.

Dear Luca

You can perform HPTLC using these

Thin layer chromatography (TLC-silica-gel) was performed on cold and soxhlet ethanol-water extracts. The solvent system used was chloroform (6.5)-methanol (3.5)--water (1.0). The plate was developed with Sakaguchi reaaent which gives a reddish-orange colour with galegine.

Do you know how we can obtain the standard sample of the Galega for HPLC quantification? 

Dear Ali,

you can purchase it from Akos Consulting & Solution GmbH (http://www.akosgmbh.de/AKosSamples/index.html).

Product Code should be: AKOS000276789 500mg - stock