How do I purify cell wall extract from mycobacterium culture?

Abazar Razavinia @Abazar-Razavinia

14 December 2015 1 4K Report

how do i make sure that only cell wall is purified after sonication and centrifuge

Taghreed Hafiz

You could do this through membrane preparations as described by Fisher et al. (Fisher et al., 2009).

how do i make sure that only cell wall is purified after sonication and centrifuge

Use the UV-visible spectroscopic analysis of freshly prepared membrane to record the spectra

Good Luck

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