Hi Kodwo.

The kidney is a tough tissues consisting of many tubular structures for the dissociation into single cell suspension. I would suggest the use of mortar and pestle after snap freezing small chopped tissue pieces of the kidney by plunging into LN2 gas for 5 min. Take them into chilled mortar and pestle (at -70C for 1 hour). Wear your thick gloves, and grind them completely to obtain tissue powder. You may need additional LN2 to be poured to make powder without making aggregated clumps.

Take a small amount of the powder (known weight >5~10mg) into a known vol of lysis buffer. Do the remaining routine for your protein contents and make SDS-sample-buffered kidney proteins.

Good luck.

Thank you very much. I will give up feedback when I am done with your procedure.

Thank you once again.