Hello Can anyone suggest a good way of inducing oxidative stress in mice to exhibit proteinurea phenotype in the mice?

Hello Can anyone suggest a good way of inducing oxidative stress in mice to exhibit proteinurea phenotype in the mice or a good method to induce oxidative stress in the mice to study kidney...

26 July 2020 2,999 3 View

Why does my freshly thawed and seeded HeLa cells look different than usual?

I had stored HeLa cells in -80C freezer for over a month. When I thawed and seeded the cells today, I noticed that the cells looked significantly different than usual cells when floating in media....

25 November 2019 4,352 6 View

How can I strip off bound antibody from live cell?

I have labeled MCF-7 cells with a certain cell surface antibody and I want to unconjugate the antibody from the cell surface without affecting the cell viability.

20 August 2019 2,426 2 View

Power as boundary condition?

Hello Everyone, I have a pipe from which some fluid is flowing. At the outside wall of a pipe, I have some electrical devices that are touching the pipe from the outside. The power...

10 April 2019 4,099 3 View

How Carrier and Code NCO generates new frequency and phase?

Hello Everyone, Can someone please explain me how the output coming out of PLL or DLL loop filter is utilised by the NCO to generate new frequency using the look-up table. I have read it in...

17 June 2018 8,847 2 View

What should be the appropriate age and weight of albino rats for inducing Type 2 Diabetes ? Also, which albino rat is more appropriate, Wistar or SD?

This is regarding my research on effectiveness of my treatment on type 2 induced albino rats

22 September 2017 5,424 2 View

How to process fish roe to get proper and visible band in 1D SDS-PAGE?

I have free-dried the roe and after that removed the oil from dried roe using iso-propanol , followed by grinding and sieving to get roe-protein concentrate (RPC),The aqueous extract of the RPC...

19 July 2017 7,754 2 View

How can I make cross sections of honey bee brains?

16 May 2016 6,162 7 View

What could be the possible reason of decreasing XRD intensity with thermal annealing?

In some papers I found XRD intensity of some materials in thin film form decreases with thermal annealing. That means crystallinity decreases ? I am not able to find the proper reason for it.

09 May 2013 2,887 19 View

How does crystallite size vary with peak intensity of XRD pattern?

If XRD peak intensity of a thin film increases with respect to some external parameters, then what will happen to the crystallite size? Is there any formula to relate peak intensity with...

13 April 2013 5,820 18 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View