I want to assess the radical scavenging activity of plants extracts using the DPPH method. My protocol is stating that I need to prepare 0.1 mM of DPPH in methanol. However I don't have the reagent itself. What is available right now is a prepared solution of DPPH at 0.1%.
How can I prepare my solution of 0.1 mM OF DPPH from the 0.1% DPPH solution?
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Faida Khadidja Idaroussi Hope this previous discussion help you:
https://www.researchgate.net/post/Which-is-the-right-for-01mM-DPPH-Solution-preparation
394 g/mol is the molecular weight of DPPH , So to prepare a 0.1 mM solution , you should dissolve 3.94 mg of DPPH /100ml ethanol
the 0.1 % is expressed as 0.1 g/100ml , this is equal 2.5 mM. you can dilute 2.5 to get 0.1 mM
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