Insect Lipase Activity?

Hi everyone! My name is Nacho. I am trying to assay lipase activity from insect midgut supernatant, and I have some problems and some doubts about it. I do not have the pH-stat. Thus, the...

22 November 2020 5,595 3 View

Is Tetrabutylammonium hydroxide Mass Spectrometry compatible?

Hello forum, I would like to know if TBA.OH is volatile in mixtures of acetonitrile or methanol. I want to analyze peptides that were previously dissolved in TBA.OH-conatining solution, but dried...

05 October 2020 6,936 3 View

Any good manual for phenomenological approach to sustainability?

I'm thinking about a turn from my ethnographic approach to a phenomenological approach in the study of sustainability and forest preservation. However, I didn't find any good manual to design and...

06 July 2020 9,618 1 View

Can RL agent's decision be explained after inference?

I would like to know if there is a method, or sets of method that can explain why a RL agent has chosen certain decision, after the agent has been trained. I'm not looking for different RL...

23 April 2020 151 3 View

Can you desalt peptide digests with high pH RPLC?

Hello! I am working with some materials that require desalting of the peptide samples prior testing. My samples contain a salt of potassium. With a standard sample, I would use R3 or C18 resins...

19 January 2020 6,772 3 View

Methods available to calculate animal density by means of camera trapping?

We have two large camera trap data sets (tropical and temperate forest), which I would like to use for estimating animal density. Which are the available methods? Strengths and weaknesses? Thanks

16 January 2019 5,879 3 View

I have to remove RNA from DNA samples. Using a RNAase A (4 mg/ml), which volumen do you recommend to use?

I have some sample that I have to re-purify and some samples to isolate DNA de novo. In both cases I have to use RNAase to remove RNA. I tried with 1.5 ul of RNAase A (4 mg/ml) but the RNA is...

13 December 2018 860 2 View

Available shapefile of marine sea temperature hotspots?

Dear researchers, someone has a shapefile of marine sea temperature hotspots worlwide desribed in Hobday & Pecl (2014). Thanks in advance for sharing. Regards Ignacio

17 October 2018 7,500 1 View

Which tail occluder size is appropiate for C57 mice?

We're planning to measure non invasive blood pressure in C57 mice. Weight should be around 20-40 Grams. There are 2 sizes available at Kent Scientiffic: small and extra-small... they're both...

26 June 2018 9,849 1 View

Seminal papers about digital platforms?

25 June 2018 6,246 2 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View