I used SPE columns to cleanup 1L sample volume and after eluting and drying , I reconstitute to 2ml using methanol before quantifying with HPLC-DAD?
I use SPE columns to cleanup 1L sample volume and after eluting and drying , I reconstitute to 2ml using methanol before quantifying with HPLC-DAD?ume 1L and the final reconstituted volume(2ml)?
Please I would appreciate the laboratory procedure for conducting recovery studies
Thanks in advance for your swift replies.
This is a routine component in analytical method development. Essentially you just add a known amount of analyte to the crude sample matrix and after all sample preparation procedures (SPE, drying, etc) you measure the actual amount and compare it with the original amount to get the recovery rate.
What compounds are you going to analyze? because the first thing is to filter the sample. Filters like Nylon retain some Organic components, in that case It`s necessary to use methanol for filter cleaning. Then pre-concentration is used.
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