I am filtering my protein sample through 0.2um filter as preparation for injection onto size exclusion column. The column specification suggests that the sample shall be no more than 500uL.
The filter I used was Thermo Scientific's Nalgene™ Syringe Filters - 25mm Diameter, which is recommended for 10~50mL solution. No wonder when I filtered my 500uL sample through the filter, a great amount was lost on the filter.
Do any one have suggestions on what filters would be suitable for this purpose?
Thanks a lot!
Ted
Dear Ted Li,
You can filter small amount of solution by centrifuge tube filter provided by many companies. You may be follow the links. Cellulose acetate (CA) membranes provide fast flow rates and low protein binding.
Regards,
http://www.sigmaaldrich.com/catalog/product/sigma/cls8160?lang=en®ion=IR
http://www.chromtech.com/Catalog7/PDF_Indvpge/282.pdf
Ted
You could use Millex 0.22 microns filter from Millipore Corporation -some volume loss will be there even with these so you may recover 450 microlitre or so which is OK as forcing the sample out will cause frothing which is not good
Hi Ted,
As Gautam Krishnan you can try to find the proper filter on Merk Millipore website. They sell you filter with low volume retention (such as the very small 4mm filters) and with membrane made with different materials so you can reduce protein adsorption into the filter.
Good luck
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