Ted Li

2 Questions 2 Answers 0 Followers

Questions related from Ted Li

How do I prepare EM grids (negative stain & cryo) without inducing protein aggregation?

I was preparing some negative stain EM grids for protein samples. I purified the proteins with gel filtration right before grid preparation and the traces looked very good (quite symmetric and...

11 November 2015 579 12 View

How do I minimize protein sample loss during filtration?

I am filtering my protein sample through 0.2um filter as preparation for injection onto size exclusion column. The column specification suggests that the sample shall be no more than 500uL. The...

01 January 2015 3,334 3 View