Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

Can I use breaking series as a regressor in a VAR model?

Context: I have two series, price and sales. Sales is mean-reverting stationery but price is stationary only after controlling for an intercept break. I want to set up a 2-equation VAR model...

14 January 2021 6,824 12 View

Does the use of Tissue Level implants reduce the risk of Peri-implantitis, as opposed to using bone level?

12 December 2020 6,007 6 View

Will the plasmid without SV40 Ori have a quite low expression level in 293T?

SV40 Ori helps plasmid to replicate in 293T cells. If I use a plasmid which doesn't have a SV40 Ori, will the expression level be very low?

10 December 2020 1,751 2 View

How to calculate the normalized frequency V of photonic crystal fiber using COMSOL?In particular, how to calculate NEFF and nFSM using COMSOL?

24 November 2020 967 2 View

HOW to do an intention-to-treat analysis(ITTA)?

I mean, how do I put the dropped out participants' outcome (Missing value) in my analysis? Although I have already known what's ITTA meaning, I still don't know how to do it. Which methods should...

23 November 2020 9,982 5 View

Does pEGFP plasmid have G418 resistant?

Instruction from pEGFP: The recombinant EGFP vector can be transfected into mammalian cells using any standard transfection method. If required, stable transformants can be selected usingG418 ....

20 October 2020 4,662 2 View

The solubility of ethinyl estradiol (EE2) in methanol?

What is the solubility of ethinyl estradiol (EE2) in methanol? In the experiment, methanol was used to help dissolve, what is the reason for the flocs appearing after the storage solution is added...

20 September 2020 4,055 1 View

How to compare methylation status between different cell lines?

As in the title. I got the methylation status of several genes in different cell lines by bisulfite sequencing. More specifically, I have the sequencing data from the amplicons of these genes...

30 August 2020 6,020 2 View

How can I digest the formaldehyde (1%) crosslinked DNA-protein with restriction enzyme (DpnII) better?

I am working on yeast samples for 4C-seq study , but the formaldehyde fixed samples can not be digested with restriction enzyme (DpnII). Please help me out. Thanks.

28 August 2020 6,483 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

Phosphate buffer with different pH conditions: HCl or NaH2PO4?

Hi all, I just encountered this dogma; when adjusting pH of phosphate buffers, I've been using HCl/NaOH to in/decrease pH of buffers and recently I was told that HCl/NaOH can't be used to adjust...

08 February 2021 9,070 5 View

Why am I getting such large heats of dilution in my Isothermal Titration Calorimetry Experiment?

I am trying to measure binding of calcium to an expressed protein we are studying and am getting very large heats of dilution for the calcium solution in my control tests. We prepared a 1 M CaCl2...

07 February 2021 7,532 1 View

Why FTIR can not detect bases of nucleic acid in extracellular polymeric substances (EPS)?

I characterized functional groups of EPS using FTIR. Goups of sugars and proteins (e.g., -OH, amide, -COOH) as well as phosphate group of nucleic acid were readily detected. However, peaks of...

05 February 2021 4,405 1 View

Why sometimes current- voltage plot in chemiresistors/ FET does not pass through the origin?

I am trying to measure resistance from I-V curve from a chemiresistor/ FET sensor with a keithly sourcemeter. The substrate is soaked/ wet with protein and phosphate buffer only. But the I-V line...

24 January 2021 8,009 3 View

How can you accurately induce calcium overload and measure membrane potential in cultured Tg2576 neuronal cells using whole cell patch clamp method?

Hi there, I'm currently working on a project where i need to design an experiment but as i have little to no experience with this level of experimental design and no face to face help i am really...

22 January 2021 6,728 3 View

Why calcium oxide nanoparticles becomes black at 650 degree temp?

Please tell me, which temp can I select for neglect black color of the Calcium oxide nanoparticles ..

14 January 2021 5,341 5 View

How do I justify the use of fish scales to synthesize a nano filter?

Good day. I am doing a studu on the use of fish scales to create a nano filter. This is aimed at potentially solving the face mask problem brought about the pandemic. This is what I know so...

09 January 2021 8,907 5 View