I have prepared methanol extract of a tree bark. From that i have to isolate particular compounds. Could you guide me how to isolate. The compounds are
(1) 2,5dimethyl-1,2,3,4-tetrahydropyrimido[1,6-A]Indol-7-yLmethylether
(2) phenol,2-4-bis(1,1-dimethylpropyl)
(3) 7-acetoxy-9,10-dihydrobenzo[a]pyrene
(4) 2-methyl-6,7-dimethoxy-4-quinolinol
These compound names are obtained from GC-MS results.
GC MS has already given you an idea of the type of compounds in the isolate you gave for analysis - what do you want to go for a isolation again for?
Normally we isolate active compounds. Now as you know the composition of your extracts, you should first check their pharmacological activity and if they are found active in vitro or in vivo, you may decide on isolation. Methanol extract from bark would be containing phenolic compounds in particular. May be you should try to isolate the major compound.
As a previous researcher, why do you want to isolate them if you know what are they?. Any way, I suppose your plant has been studied before, why don't you do a bibliographic research, find papers about it and look at how they have worked, and finally you should use CC to isolate them. Good luck.
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