I haven't been able to find the actual forms for either of these tools that contain both the questions and scales. I've found the questions, but not the values of the Likert scales used.
I am trying to optimize my control titration for ITC so that I can ensure my ligand to protein traces are reliable. My small molecule is stored in DMSO. I have been very careful to avoid buffer...
14 February 2024 9,147 3 View
Hello, I have recently switched from doing ligand into protein for ITC because my ligand is in DMSO and has low solubility at 10% DMSO; therefore, now I am doing protein into ligand -- the heat...
09 February 2024 4,680 1 View
Hello, I am having an ITC issue because I cant increase my ligand concentration due to solubility issues. I know there is past literature on reverse titrations being acceptable. I am more asking...
06 February 2024 5,564 2 View
Hello, I am doing ITC and getting repeatable curves that look very promising. After reading some articles, it seems the biggest challenge to avoid is buffer mismatch. Is there a way to rule out...
18 January 2024 1,224 5 View
Hello, I have had moderate success with ITC but most of my issues have been with the Nano Program in fitting. I have a clear curve but the program is not fitting it correctly, does anyone have a...
22 December 2023 9,331 1 View
Hello, I am doing a titration of ligand into protein and am getting good heat releases compared to my ligand into buffer. I am getting more heat release in later injections. Could cooperativity...
07 November 2023 775 4 View
I would like to know how long it takes to get access after requesting permission from authors for articles.
13 October 2023 530 2 View
Dear Colleagues, Title of Research Project: Exploring Reflective Learning Strategies in Big Data Analytics Education and Practice: A Cross-sectional Study on Adoption, Effectiveness, and...
02 October 2023 3,652 2 View
Electromagnetic waves with frequencies approaching those of a plasma with a similar election plasma frequency, are highly reflected/absorbed by the plasma and can be predicted by a dispersion...
17 August 2023 2,737 0 View
I am capturing fluorescent microscopy images of a developmental process with a high degree of variability. I need to compile these images into a "gallery" so that I can see many/all images at once...
26 July 2023 3,140 3 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
EDX analisys
31 July 2024 5,947 2 View
I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...
29 July 2024 8,034 4 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...
26 July 2024 6,606 0 View
"Integration of Mass Media and Communication into the System of International Relations: Place, Role, Actuality and Prospects: Modern Media Issues 2024" Website: https://immcir.alnoor.edu.iq/
23 July 2024 3,139 6 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View