Hi folks,

I would like to use REST2009 to analyse my qPCR data. Is there any way to get the underlying raw data from the calculated expression values out of the program to do analysing elsewhere or just do plot different graphs? I understand that the calculations are based on the modified Livak-method and when I perform them manually in an excel-sheet as decribed here

https://www.researchgate.net/post/Advice_on_comparative_CT_qPCR_analysis_applied_to_environmental_samples

I get the same expression levels. However, I don't know how to perform statistics on these calculations because you only get single values. I would be very greatful if somebody could help.

Best regards

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