How do I design or find a kit that helps to measure ATGL (Adipose Triglyceride Lipase) activity?

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Adaku C Ume

Erin, you are amazing! Thank you for that idea!!! I will ask my PI and see if he doesn't mind me doing this!!!

Apurva Kumar

I assume you are looking at the HSL; in that case you can measure free fatty acid liberated by enzymatic hydrolysis of a triglyceride emulsion in the absence of heat inactivated serum (creates absence of apoCii). You can use a copper estimating compound after forming copper complex of free fatty acid using Cu(NO3)2. Of course u will have to extract free fatty acids into chloroform. Will try to get a ref for you.