Does anyone know where to get phospho-ATGL antibodies to determine ATGL activity?

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Jan Novakofski

Antibodies will enable you to measure protein, but unfortunately will not provide any information on enzyme activity. If you in fact want a function assay (=activitiy) I would guess you could modify a classic HSL assay perhpas by HSL nulling ABs.

Adaku C Ume

Thank you for your help but My assignment requires that I measure ATGL not HSL activity and there are no kits measuring this enzyme activity. Phosphorylation is strongly represents activity since phosphorylation is required for activity. Measuring the amount of phosphorylated ATGL will indicate its activity. I appreciate your help!!!!