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Hey, does anyone know any effective way to separate virus from exosomes fraction? my problem with the separation of the EVs in this system is the fact that the virus that we work with particles...

06 December 2020 4,765 2 View

Number of cells for extacellular vesicles extraction?

hello! I want to extract vesicles from MDA MB 231 cell ligne, culture in serum free media for 48h, would you help me with th number of cells required to have the vesicles? thank you

03 December 2020 6,488 2 View

What are the XRD analysis conditions of the cement, sand and Limestone ?

I am a Phd student and I need to do XRD analysis on my pouder samples namely ( cement- sand- limestone and calcined clay). I was asked asked to specify the analysis conditions: -the nature of...

07 October 2020 5,737 6 View

Can anyone recommend me some articles about Fire endurance of wood concrete?

Are there documentations about Fire endurance of wood or wood shavings concrete? The literature is not very clear about this .

12 August 2020 8,203 3 View

How do I quantify components of a plant crude extract?

The quedtion aims to seek help on information or literature on how to quantify the amount of metabolite(s) in a crude plant extract of dried Calotropis procera leaves. I wish to determine by...

14 June 2020 3,610 8 View

Is it possible to use plant extract as natural sterilizer?

For economic reasons, sterilzation against covid_19 is very necessary, so is it possible to use plant extract?

11 May 2020 2,674 14 View

Which statistical analysis is more appropriate for my study?

I am trying to find the relationship between the use of defence mechanisms in early adulthood as predictors of neuroticism. Those who use more defence mechanisms will score higher on the...

14 April 2020 3,353 7 View

Preparation of the mix for Standard PCR (without using a kit)?

Hi, Please anyone already prepared the mix for Standard PCR? But without using a commercial kit. It is for staphylococcal enterotoxins genes. I am looking for the concentrations of each...

08 February 2020 4,487 6 View

How to deal with mental health issues?

I feel communication helps in coping such issues to some level.

16 August 2019 7,405 31 View

Course -Preparation advices !?

Dear colleagues, As I am new in the domain of teaching courses, so it takes too much of time to prepare my course, saying for example: cryptography course, web development,..etc,... So, my...

30 May 2019 6,236 3 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

How can we add reinforced thermoplastics with granules in injection molding machine?

I need to make an injection molding machine in which short fibers are to be introduced. Short fiber length and material properties are to be determined and also the material strength are to be...

22 February 2021 4,290 1 View

Why is my qPCR suddently not working?

I've been working with qPCR for some months now. I amplify 2 genes (PSEN2 and HN1), and the protocols are optimized for both and have been working fine. The other day I amplified HN1 for some...

21 February 2021 3,437 3 View

Drop weight impact testing machine for composites?

Dear all, I hope everyone is safe and healthy I do have a question please. Where can I find a drop-weight impact testing machine to test fiber reinforced polymer composite plates (CFRP and GFRP)...

17 February 2021 3,820 3 View