Hi all!
I have the output of amplicon sequencing data (18S) for algae species from two sites. I am looking for a method that is not TOO complex where I can analyse which species are correlated with the presence of my target species.
Thanks in advance.
by saying correlation do you mean that which are the other organisms present in the two samples apart from your target organism?
For analyzing amplicon sequencing there are quite a number of programs and pipelines (QIIME2, MOTHUR) but this requires special environment, knowledge of Linux based languages and are a bit complex.
The easiest method you can use is the GAIA platform (https://metagenomics.sequentiabiotech.com/). It is user-friendly, more accurate and fast. you can use it for free up to 2GB of data. if you have more than 2GB of data then you need to avail their paid services.
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